Summary
Type: SPE
Application #: 301370
Catalog#: 730024 - SPE Cartridges, Chromabond, C8, 6ml, PP, 500mg, 30/pk
Detection:
Conditions: Column conditioning:
Slowly force or aspirate 5 ml n-hexane through the column, dry the column for
30 sec under vacuum, then force or aspirate 1 column volume methanol through
the column; now the column must not run dry
Sample application:
Force or aspirate 250 μl sample solution through the column
Column washing:
Apply 1 ml acetonitrile/water (7 : 3, v/v) to the column, wait 30 sec; then
vacuum dry column 10 min
Elution: 3 x 500 μl n-hexane
Authors: LC Department
Source: Macherey-Nagel